Products

• Proprietary Systems for Improved Assay Performance
• Intellectual Property
• Data for Human Health Management
• Clinical Applications

Proprietary Systems for Improved Assay Performance

Anteo Diagnostics specialises in simple to use systems that immobilise components of biological assays, such as antibodies, on synthetic substrates without loss of functionality. These systems can be applied to various commonly used test formats such as microscope slides, beads, porous membranes and microtiter plates.

Existing methods of immobilisation, such as passive coating or covalent coupling, damage protein structure and function. In contrast, Anteo’s systems are designed to interact with specific regions of a protein, giving assay uniformity and reproducibility. This in turn allows optimum assay sensitivity with less reagent and sample required, faster reaction times, and improved multi-analyte data analysis and interpretation. See Fact Sheet for more information.

These proprietary systems underpin Anteo Diagnostics’ capability for rapid development of better solid phase assays for its partners. Two of the systems, Mix&Go™ and Mix&Select, can be made available to other test developers.

Mix&Go™

Figure: Mix&Go™ uses a range of metal structures to determine binding location and overall binding strength.

Mix&Go™ can be simply applied to many different substrates and once applied, is stable and can be stored with no loss of performance.  However, once some protein is added, such as an antibody, it binds the antibody to the synthetic substrate in a matter of minutes.  After washing off excess antibody, it is ready for performing assays.

Mix&Select

The Mix&Select family uses a combination of two or more weaker binding components to improve the specificity of the assay.

Figure: Mix&Select utilises multi-component binding of weak/medium affinity ligands to immobilize proteins on surfaces. There are small molecule ligands with good specificity but poor binding strength, metal complexes with opposite characteristics, and polymeric surface coatings to control background.

The main advantage of Mix&Select over Mix&Go™ is the use of highly selective components to capture specific proteins in preference over others. For example, Mix&Select surfaces can be specifically designed to selectively bind antibodies in the presence of other proteins such as human serum albumin in blood samples.